Bacteria in Spacecraft Clean Rooms Can Go Dormant, Evading Death

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Bacteria in Spacecraft Clean Rooms Can Go Dormant, Evading Death
A type of bacteria found in clean rooms has an unexpected method of survival, with implications for planetary protection
Stephanie Pappas
Scientific American
Updated Thu, September 4, 2025 at 6:30 AM EDT
3 min read



NASA's Curiosity rover is prepared for launch in the clean room at the Spacecraft Assembly Facility at NASA's Jet Propulsion Laboratory, Pasadena, CA.


A bacterial species found in spacecraft clean rooms can survive intensive antimicrobial cleaning by going dormant, new research finds. That’s important because other clean-room survivors had been known to live through disinfection by forming spores, which are thick-walled structures that protect bacteria from high temperatures or toxins such as ethanol. The actinobacterium Tersicoccus phoenicis can’t form these spores, but a new study published in the journal Microbiology Spectrum shows that it can go into a state similar to hibernation. In this state, it has no growth and almost no metabolism but has the ability to “wake up” when conditions improve.

“In the cleanest places we build—spacecraft, pharma plants, food facilities—some microbes aren’t dead: they’re dormant,” says Alberto G. Fairén, an astrobiologist at Cornell University who wasn’t involved in the research.

While in this dormant state, T. phoenicis can’t be detected by the usual method of swabbing surfaces and checking which bacteria grow in culture from the swabs. That means it could theoretically sneak aboard spacecraft that are supposed to be free of Earth contaminants. If such a bug hitched a ride to another planet, it could wake up upon arrival and potentially disrupt existing extraterrestrial life. “It’s a huge planetary protection concern,” says Madhan Tirumalai, a biologist and biochemist at the University of Houston and lead author of the new study.

T. phoenicis was first discovered in a clean room at NASA’s Kennedy Space Center in Florida, where the Mars lander Phoenix was being prepared for launch. Two years later it popped up in a European Space Agency clean room in South America. In 2013 scientists discovered that this mystery survivor was not only a new species but a new genus of bacteria.

This species is part of a larger group of bacteria, known as actinomycetota or actinobacteria, that are able to go dormant when conditions aren’t conducive to growth. (One famous member of this group is Mycobacterium tuberculosis, the bacterium that causes tuberculosis, which can go dormant and persist in the lungs over a lifetime.) To learn whether T. phoenicis was capable of dormancy, Tirumalai and his colleagues deprived cells of nutrients and extracted all water from them (a process called desiccation). The cells stopped growing, and the number of viable cells plummeted within days.

To show that these nonviable cells were dormant, not dead, the researchers added a protein called a resuscitation-promoting factor (Rpf), which is known to “wake up” other species of dormant actinobacteria. The Rpf revived the cells, “proving they were alive but silent,” Tirumalai says.

That’s a concern for human travel to a place such as Mars, which could offer a new, nutrient-rich environment to the hibernating microbes. Astronauts trying to survive on the red planet would need to grow food, and the sugars and nutrients involved could revive the bacteria, says study co-author William Widger, a University of Houston biologist. “That would be in the environmental safe quarters of astronauts, probably where you’d not want them.”

The microbe likely couldn’t survive on the Martian surface, however, Fairén says. “The high UV flux, extreme cold and desiccation, low atmospheric pressure, and cosmic radiation on Mars are overwhelmingly hostile—even to spore-formers. Dormant nonspore, nonprotected cells would almost certainly not endure long on exposed surfaces on Mars—minutes or less.”

That makes contamination from a robotic mission an unlikely concern, Fairén says, although human missions to the planet will almost certainly contaminate it. The paper does highlight the need for better detection and targeting of non-spore-forming bacteria in clean rooms, he says.

It’s not yet clear how to effectively clean up dormant microbes. Tirumalai and his colleagues are now looking to test other clean-room survivors for their dormancy potential, which would make a case for upending current cleaning procedures.

“If we can show that a significant number of these organisms that have been isolated from clean rooms can go into dormancy,” Tirumalai says, “bingo—we have a much bigger story.”

https://www.yahoo.com/news/articles/bacteria-spacecraft-clean-rooms-dormant-103000157.html